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Progress In Electromagnetics Research
ISSN: 1070-4698, E-ISSN: 1559-8985
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EXPOSURE TO 2.45 GHz MICROWAVE RADIATION PROVOKES CEREBRAL CHANGES IN INDUCTION OF HSP-90 α/β HEAT SHOCK PROTEIN IN RAT.

By T. Jorge-Mora, M. Alvarez Folgueiras, J. M. Leiro-Vidal, F. J. Jorge-Barreiro, F. J. Ares-Pena, and E. López-Martin

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Abstract:
Physical agents such as non-ionizing continuous-wave 2.45 GHz radiation may cause damage that alters cellular homeostasis and may trigger activation of the genes that encode heat shock proteins (HSP). We used Enzyme-Linked ImmunoSorbent Assay (ELI-SA) and immunohistochemistry to analyze the changes in levels of HSP-90 and its distribution in the brain of Sprague-Dawley rats, ninety minutes and twenty-four hours after acute (30 min) continuous exposure to 2.45 GHz radiation in a the Gigahertz Transverse Electromagnetic (GTEM cell). In addition, we studied further indicators of neuronal insult: dark neurons, chromatin condensation and nucleus fragmentation, which were observed under optical conventional or fluorescence microscopy after DAPI staining. The cellular distribution of protein HSP-90 in the brain increased with each corresponding (0.034 ± 3.10-3, 0.069 ± 5.10-3, 0.27 ± 21.10-3 W/kg), in hypothalamic nuclei, limbic cortex and somatosensorial cortex after exposure to the radiation. At twenty-four hours post-irradiation, levels of HSP-90 protein remained high in all hypothalamic nuclei for all SARs, and in the parietal cortex, except the limbic system, HSP-90 levels were lower than in non-irradiated rats, almost half the levels in rats exposed to the highest power radiation. Non-apoptotic cellular nuclei and a some dark neurons were found ninety minutes and twenty-four hours after maximal SAR exposure. The results suggest that acute exposure to electromagnetic fields triggered an imbalance in anatomical HSP-90 levels but the anti-apoptotic mechanism is probably sufficient to compensate the non-ionizing stimulus. Further studies are required to determine the regional effects of chronic electromagnetic pollution on heat shock proteins and their involvement in neurological processes and neuronal damage.

Citation:
T. Jorge-Mora, M. Alvarez Folgueiras, J. M. Leiro-Vidal, F. J. Jorge-Barreiro, F. J. Ares-Pena, and E. López-Martin, "Exposure to 2.45 GHz microwave radiation provokes cerebral changes in induction of hsp-90 α/β heat shock protein in rat.," Progress In Electromagnetics Research, Vol. 100, 351-379, 2010.
doi:10.2528/PIER09102804
http://www.jpier.org/pier/pier.php?paper=09102804

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