volume | PIER Journals

Abstract

A study on the use of non-ionizing and non-thermal millimeter electromagnetic radiation in tumor chemotherapy was conducted. DNA released from sarcoma 45 tumor (tDNA) and healthy rats (hDNA) in water-saline solution was irradiated during 90 min by frequencies at both resonates for oscillations of water molecular structures (at 64.5 GHz and 50.3 GHz) and non-resonance (48.3 GHz). Non-irradiated and irradiated tDNA and hDNA binding constants with anti-tumorous drugs doxorubicin (DX) and netropsin (NT) were studied. The absorption spectra of non-irradiated and irradiated complexes of DNA with DX and NT were obtained by spectroscopic method. From the absorption spectra, binding constants at 290 K, 300 K, and 310 K temperatures have been determined. According to our calculations doxorubicin and netropsin with irradiated DNA form were more stable complexes and much stronger with tDNA irradiated at resonant frequencies: it was observed doxorubicin and netropsin binding selectivity to irradiated tDNA in-vitro experiments. For a DNA irradiation at resonant frequencies of 64.5 GHz and 50.3 GHz the binding constant K to DX and to NT is almost an order of magnitude higher than for the non-irradiated DNA. The obtained data suggest that the irradiation of malignant tumors by non-thermal (ultra-weak intensity) millimeter electromagnetic waves in combination with anticancer drugs may be promising for clinical oncology. The same antitumor effect can be achieved at much lower doses of medicines (considerable dose reduction). This is essential from the point of view of the application of gentle therapies for patients and the reduction of expenses associated with acquisition of expensive medicines.

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Dr. Vitaly P. Kalantaryan

Dr. Radik Martirosyan

Dr. Yura Babayan

Dr. Ruzanna Khazaryan