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PIER PIER B PIER C PIER M PIER Letters
2011-01-10
Inhibition of Bone Formation by High Intensity Pulsed Electromagnetic Field in Mc3t3-E1 Cells
By
Kangchu Li,

    Dr. Kangchu Li

    Department of Radiation Medicine, School of Public Health

    The Fourth Military Medical University

    China

    Homepage

Yanping Hui,

    Dr. Yanping Hui

    Department of Pathology

    Fourth Military Medical University

    China

    Homepage

Shirong Ma,

    Dr. Shirong Ma

    Department of Pathology

    Fourth Military Medical University

    China

    Homepage

Guirong Ding,

    Dr. Guirong Ding

    Department of Radiation Medicine, School of public health

    The Fourth Military Medical University

    China

    Homepage

Yao Guo,

    Dr. Yao Guo

    Department of Radiation Medicine, School of Public Health

    Fourth Military Medical University

    China

    Homepage

Junye Liu,

    Dr. Junye Liu

    Department of Radiation Medicine, Faculty of Preventive Medicine

    The Fourth Military Medical University

    China

    Homepage

Yurong Li,

    Dr. Yurong Li

    Department of Radiation Medicine, Faculty of Preventive Medicine

    The Fourth Military Medical University

    China

    Homepage

Guozhen Guo

    Professor Guozhen Guo

    Department of Radiation Medicine, Faculty of Preventive Medicine

    The Fourth Military Medical University

    China

    Homepage

Progress In Electromagnetics Research, Vol. 112, 139-153, 2011
doi:10.2528/PIER10110203
Abstract
To investigate the effects of pulsed electromagnetic field (PEMF) with high electric field intensity on bone formation in murine osteoblast-like MC3T3-E1 cells, proliferation, alkaline phosphotase (ALP) activity, mineralized nodule formation, Collagen Type I (COL-I) and core-binding factor (Cbf)a1 mRNA expression, and bone morphogenetic protein (BMP)2/4 and mothers against decapentaplegic (Smad)1/5/8 protein expression were examined in cultured MC3T3-E1 cells after exposure to PEMF at the field intensities of 0kV/m, 50kV/m or 400kV/m for 400 consecutive pulses daily for 7 consecutive days. After 50 kV/m of PEMF exposure, none of the above parameters of MC3T3-E1 cells changed significantly when compared to the control groups. However, the proliferation, ALP activity and mineralized nodule formation of MC3T3-E1 cells in 400 kV/m PEMF exposure groups decreased significantly although COL-I and Cbfa1 mRNA expression and BMP2/4 and Smad1/5/8 protein expression did not change. The PEMF we used at high electric field intensity suppressed proliferation, differentiation and mineralization of MC3T3-E1 cells in culture and appeared to be harmful for bone formation.
Citation
Kangchu Li Yanping Hui Shirong Ma Guirong Ding Yao Guo Junye Liu Yurong Li Guozhen Guo , "Inhibition of Bone Formation by High Intensity Pulsed Electromagnetic Field in Mc3t3-E1 Cells," Progress In Electromagnetics Research, Vol. 112, 139-153, 2011.
doi:10.2528/PIER10110203
http://www.jpier.org/PIER/pier.php?paper=10110203
References

1. Bassett, C. A., "Beneficial effects of electromagnetic fields," J. Cell. Biochem., Vol. 51, 387-393, 1993.

2. Walker, N., C. Denegar and J. Preische, "Low-intensity pulsed ultrasound and pulsed electromagnetic field in the treatment of tibial fractures: A systematic review," J. Athl. Train., Vol. 42, 530-535, 2007.

3. Liboff, A. R., T. Williams, Jr., D. M. Strong, R. Wistar, and Jr., "Timevarying magnetic fields: Effect on the DNA synthesis," Science, Vol. 223, 818-819, 1984.
doi:10.1126/science.6695183

4. Ozawa, H., E. Abe, Y. Shibasaki, T. Fukuhara, and T. Suda, "Electric fields stimulate DNA synthesis of mouse osteoblast-like cells (MC3T3-E1) by a mechanism involving calcium ions," J. Cell. Physiol., Vol. 138, 477-483, 1989.
doi:10.1002/jcp.1041380306

5. Diniz, P., K. Shomura, K. Soejima, and G. Ito, "Effects of pulsed electromagnetic field (PEMF) stimulation on bone tissue like formation are dependent on the maturation stages of the osteoblasts ," Bioelectromagnetics, Vol. 23, 398-405, 2002.
doi:10.1002/bem.10032

6. De Mattei, M., N. Gagliano, C. Moscheni, C. Dellavia, C. Calastrini, A. Pellati, M. Gioia, A. Caruso, and G. Stabellini, "Changes in polyamines, c-myc and c-fos gene expression in osteoblast-like cells exposed to pulsed electromagnetic fields," Bioelectromagnetics, Vol. 26, 207-214, 2005.
doi:10.1002/bem.20068

7. Icaro Cornaglia, A., M. Casasco, F. Riva, A. Farina, L. Fassina, L. Visai, and A. Casasco, "Stimulation of osteoblast growth by an electromagnetic field in a model of bone-like construct," Eur. J. Histochem., Vol. 50, 199-204, 2006.

8. Takano-Yamamoto, T., M. Kawakami, and M. Sakuda, "Effect of a pulsing electromagnetic field on demineralized bone-matrix-induced bone formation in a bony defect in the premaxilla of rats," J. Dent. Res., Vol. 71, 1920-1925, 1992.
doi:10.1177/00220345920710121301

9. Hartig, M., U. Joos, and H. P. Wiesmann, "Capacitively coupled electric fields accelerate proliferation of osteoblast-like primary cells and increase bone extracellular matrix formation in vitro," Eur. Biophys. J., Vol. 29, 499-506, 2000.
doi:10.1007/s002490000100

10. Landry, P. S., K. K. Sadasivan, A. A. Marino, and J. A. Albright, "Electromagnetic fields can affect osteogenesis by increasing the rate of differentiation," Clin. Orthop. Relat. Res., 262-270, 1997.
doi:10.1097/00003086-199705000-00035

11. Yamamoto, Y., Y. Ohsaki, T. Goto, A. Nakasima, and T. Iijima, "Effects of static magnetic fields on bone formation in rat osteoblast cultures ," J. Dent. Res., Vol. 82, 962-966, 2003.
doi:10.1177/154405910308201205

12. Aaron, R. K. and D. M. Ciombor, "Acceleration of experimental endochondral ossification by biophysical stimulation of the progenitor cell pool ," J. Orthop. Surg., Vol. 14, 582-589, 1996.

13. Aaron, R. K., D. M. Ciombor, and G. Jolly, "Stimulation of experimental endochondral ossification by low-energy pulsing electromagnetic fields," J. Bone Miner. Res., Vol. 4, 227-233, 1989.
doi:10.1002/jbmr.5650040215

14. McLeod, K. J. and L. Collazo, "Suppression of a differentiation response in MC-3T3-E1 osteoblast-like cells by sustained, low-level, 30 Hz magnetic-field exposure," Radiat. Res., Vol. 153, 706-714, 2000.
doi:10.1667/0033-7587(2000)153[0706:SOADRI]2.0.CO;2

15. Lohmann, C. H., Z. Schwartz, Y. Liu, H. Guerkov, D. D. Dean, B. Simon, and B. D. Boyan, "Pulsed electromagnetic field stimulation of MG63 osteoblast-like cells affects differentiation and local factor production," J. Orthop. Res., Vol. 18, 637-646, 2000.
doi:10.1002/jor.1100180417

16. Lohmann, C. H., Z. Schwartz, Y. Liu, Z. Li, B. J. Simon, V. L. Sylvia, D. D. Dean, L. F. Bonewald, H. J. Donahue, and B. D. Boyan, "Pulsed electromagnetic fields affect phenotype and connexin 43 protein expression in MLO-Y4 osteocyte-like cells and ROS 17/2.8 osteoblast-like cells," J. Orthop. Res., Vol. 21, 326-334, 2003.
doi:10.1016/S0736-0266(02)00137-7

17. Chang, W. H., L. T. Chen, J. S. Sun, and F. H. Lin, "Effect of pulse-burst electromagnetic field stimulation on osteoblast cell activities," Bioelectromagnetics, Vol. 25, 457-465, 2004.
doi:10.1002/bem.20016

18. Cohly, H. H., G. E. Abraham, K. Ndebele, J. J. Jenkins, J. Thompson, and M. F. Angel, "Effects of static electromagnetic fields on characteristics of MG-63 osteoblasts grown in culture," Biomed. Sci. Instrum., Vol. 39, 454-459, 2003.

19. Hannay, G., D. Leavesley, and M. Pearcy, "Timing of pulsed electromagnetic field stimulation does not affect the promotion of bone cell development," Bioelectromagnetics, Vol. 26, 670-676, 2005.
doi:10.1002/bem.20166

20. T., M. Alvarez-Folgueiras, J. Leiro, F. J. Jorge-Barreiro, F. J. Ares-Pena, Jorge-Mora and E. Lopez-Martin, "Exposure to 2.45 GHz microwave radiation provokes cerebral changes in induction of hsp-90 a/b heat shock protein in rat," Progress In Electromagnetics Research, Vol. 100, 351-379, 2010.
doi:10.2528/PIER09102804

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