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PIER PIER B PIER C PIER M PIER Letters
2011-01-10
Inhibition of Bone Formation by High Intensity Pulsed Electromagnetic Field in Mc3t3-E1 Cells
By
Kangchu Li,

    Dr. Kangchu Li

    Department of Radiation Medicine, School of Public Health

    The Fourth Military Medical University

    China

    Homepage

Yanping Hui,

    Dr. Yanping Hui

    Department of Pathology

    Fourth Military Medical University

    China

    Homepage

Shirong Ma,

    Dr. Shirong Ma

    Department of Pathology

    Fourth Military Medical University

    China

    Homepage

Guirong Ding,

    Dr. Guirong Ding

    Department of Radiation Medicine, School of public health

    The Fourth Military Medical University

    China

    Homepage

Yao Guo,

    Dr. Yao Guo

    Department of Radiation Medicine, School of Public Health

    Fourth Military Medical University

    China

    Homepage

Junye Liu,

    Dr. Junye Liu

    Department of Radiation Medicine, Faculty of Preventive Medicine

    The Fourth Military Medical University

    China

    Homepage

Yurong Li,

    Dr. Yurong Li

    Department of Radiation Medicine, Faculty of Preventive Medicine

    The Fourth Military Medical University

    China

    Homepage

Guozhen Guo

    Professor Guozhen Guo

    Department of Radiation Medicine, Faculty of Preventive Medicine

    The Fourth Military Medical University

    China

    Homepage

Progress In Electromagnetics Research, Vol. 112, 139-153, 2011
doi:10.2528/PIER10110203
Abstract
To investigate the effects of pulsed electromagnetic field (PEMF) with high electric field intensity on bone formation in murine osteoblast-like MC3T3-E1 cells, proliferation, alkaline phosphotase (ALP) activity, mineralized nodule formation, Collagen Type I (COL-I) and core-binding factor (Cbf)a1 mRNA expression, and bone morphogenetic protein (BMP)2/4 and mothers against decapentaplegic (Smad)1/5/8 protein expression were examined in cultured MC3T3-E1 cells after exposure to PEMF at the field intensities of 0kV/m, 50kV/m or 400kV/m for 400 consecutive pulses daily for 7 consecutive days. After 50 kV/m of PEMF exposure, none of the above parameters of MC3T3-E1 cells changed significantly when compared to the control groups. However, the proliferation, ALP activity and mineralized nodule formation of MC3T3-E1 cells in 400 kV/m PEMF exposure groups decreased significantly although COL-I and Cbfa1 mRNA expression and BMP2/4 and Smad1/5/8 protein expression did not change. The PEMF we used at high electric field intensity suppressed proliferation, differentiation and mineralization of MC3T3-E1 cells in culture and appeared to be harmful for bone formation.
Citation
Kangchu Li, Yanping Hui, Shirong Ma, Guirong Ding, Yao Guo, Junye Liu, Yurong Li, and Guozhen Guo, "Inhibition of Bone Formation by High Intensity Pulsed Electromagnetic Field in Mc3t3-E1 Cells," Progress In Electromagnetics Research, Vol. 112, 139-153, 2011.
doi:10.2528/PIER10110203
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