volume | PIER Journals

Abstract

We describe an experimental set-up for exposure of small animals to radiofrequency standing waves that allows direct measurement of the power absorbed by the animal. Essentially, the set-up consists of a metallic box containing an antenna and experimental animal immobilized in a methacrylate holder; a signal generator feeding the antenna; and a power meter. In addition, the box can also contain a video camera to record the animal's behaviour, and a receiving antenna (connected externally to a power meter and a spectrum analyser) to detect alien radiation and harmonics. The absorbed power measurement trivially allows calculation of whole-body mean SAR from the animal's weight; and assuming local SARs to be proportional to whole-body mean SAR, the latter can be used to adjust organ-specific SAR predictions obtained by simulation using a commercial FDTD program with a numerical phantom. The use of the system is illustrated by application to rats given subconvulsive doses of picrotoxin to induce a seizure-prone state analogous to epilepsy: levels of the neuronal activity marker c-Fos in the frontal and piriform cortex of picrotoxin-treated rats exposed to 900 MHz GSM radiation were twice as high as those of unexposed animals.

1. Kuster, N., V. Berdinas Torres, N. Nikoloski, M. Frauscher, and W. Kainz, "Methodology of detaild dosime-try and treatment of uncertainty and variations for in vivo studies," Bioelectromagnetics, Vol. 27, 378-391, 2006.
doi:10.1002/bem.20219 Google Scholar

2. Moros, E. G., W. L. Straube, and W. F. Pickard, "A compact shielded exposure system for the simultaneous long-term UHF irradiation of forty small mammals: I. Electromagnetic and environmental design," Bioelectromagnetics, Vol. 19, 459-468, 1998.
doi:10.1002/(SICI)1521-186X(1998)19:8<459::AID-BEM3>3.0.CO;2-N Google Scholar

3. Swicord, M., J. Morrissey, D. Zakharia, M. Ballen, and Q. Balzano, "Dosimetry in mice exposed to 1.6 GHz microwave in a carrousel irradiator," Bioelectromagnetics, Vol. 20, 42-27, 1999.
doi:10.1002/(SICI)1521-186X(1999)20:1<42::AID-BEM6>3.0.CO;2-T Google Scholar

4. Moros, E. G., W. L. Straube, and W. F. Pickard, "Compact shielded exposure system for the simultaneous long-term UHF irradiation of forty small mammals: II. Dosimetry," Bioelectromagnetics, Vol. 20, 81-93, 1999.
doi:10.1002/(SICI)1521-186X(1999)20:2<81::AID-BEM2>3.0.CO;2-2 Google Scholar

5. Zygiridis, T. T. and T. D. Tsiboukis, "Assessment of human head exposure to wireless communication devices: Combined electromagnetic and thermal studies for diverse frequency bands," Progress In Electromagnetics Research B, Vol. 9, 83-96, 2008.
doi:10.2528/PIERB08071106 Google Scholar

6. Schonborn, F., K. Pokovic, and N. Kuster, "Dosimetric analysis of the carousel setup for the exposure of rats at 1.62 GHz," Bioelectromagnetics, Vol. 25, 16-26, 2004.
doi:10.1002/bem.10153 Google Scholar

7. Burkhardt, M., Y. Spinelli, and N. Kuster, "Exposure setup to test effects of wireless communications systems on the CNS," Health and Physics, Vol. 73, No. 5, 770-778, 1997. Google Scholar

8. Koester, P., J. Sakowski, W. Baumann, H. W. Glock, and J. Gimsa, "A new exposure system for the in vitro detection of GHz field effects on neuronal networks," Bioelectrochemistry, Vol. 70, No. 1, 104-114, Jan. 2007.
doi:10.1016/j.bioelechem.2006.03.039 Google Scholar

9. Hossmann, K. A. and D. M. Hermann, "Effects of electromagnetic radiation of mobile phones on the central nervous system," Bioelectromagnetics, Vol. 24, 49-62, 2003.
doi:10.1002/bem.10068 Google Scholar

10. Huber, R., V. Treyer, J. Schuderer, T. Berthold, A. Buck, N. Kuster, H. P. Landolt, and P. Achermann, "Exposure to pulsemodulated radiofrequency electromagnetic fields affects regional cerebral blood flow," European Journal of Neuroscience, Vol. 21, 1000-1009, 2005.
doi:10.1111/j.1460-9568.2005.03929.x Google Scholar

11. Zhu, Y., F. Gao, X. Yang, H. Shen, and X. Liu, "The effect of microwave emission from mobile phones on neuron survival in rat central nervous system," Progress In Electromagnetics Research, Vol. 82, 287-298, 2008.
doi:10.2528/PIER08022813 Google Scholar

12. Lin, J. C., "Cellular telephone radiation and electroencephalograms (EEG) of the human brain," IEEE Antennas and Propagation Magazine, Vol. 45, No. 5, 150-153, 2003.
doi:10.1109/MAP.2003.1252818 Google Scholar

13. Lopez-Martın, E., J. L. Relova-Quinteiro, R. Gallego-Gomez, M. Peleteiro-Fernandez, F. J. Jorge-Barreiro, and F. J. Ares-Pena, "GSM radiation triggers seizures and increases cerebral c-Fos positivity in rats pretreated with subconvulsive doses of picrotoxin," Neuroscience Letters, Vol. 298, 139-144, 2006.
doi:10.1016/j.neulet.2005.12.082 Google Scholar

14. Nutt, D. J., P. J. Cowen, C. Batts, D. G. Grahame-Smith, and A. R. Green, "Repeated administration of subconvulsant doses of GABA antagonist drugs: I. Effect on seizure threshold (kindling)," Psychopharmacology, Vol. 76, 84-87, 1982.
doi:10.1007/BF00430762 Google Scholar

15. Morgan, J. I. and T. Curran, "Stimulus-transcription coupling in the nervous system: Involvement of the inducible proto-oncogenes fos and jun," Annu. Rev. Neurosci., Vol. 14, 421-451, 1991.
doi:10.1146/annurev.ne.14.030191.002225 Google Scholar

16. Willoughby, J. O., L. Mackenzi, A. Medvedev, and J. Hiscock, "Distribution of fos-positive neurons in cortical and subcortical structures after picrotoxin induced convulsions varies with seizure type," Brain Res., Vol. 683, 73-87, 1995.
doi:10.1016/0006-8993(95)00366-X Google Scholar

17. Schmid & Partner Engineering AG. 2006 "Reference manual for the SEMCAD simulation platform for electromagnetic compatibility, antenna design and dosimetry,". Google Scholar

18. Fritze, K., C. Wiessner, N. Kuster, C. Sommer, P. Gass, D. M. Hermann, M. Kiessling, and K. A. Hossmann, "Effects of GSM microwave exposure on the genomic response of the rat brain," Neuroscience, Vol. 81, 627-639, 1997.
doi:10.1016/S0306-4522(97)00228-5 Google Scholar

19. Mickley, G. A., B. L. Cobb, P. A. Mason, and S. Farrell, "Disruption of a putative working memory task and selective expression of brain c-fos following microwave-induced hyperthermia," Physiol. Behav., Vol. 55, No. 6, 1029-1038, 1994.
doi:10.1016/0031-9384(94)90384-0 Google Scholar

20. Morrissey, R. W., S. Raney, E. Heasley, P. Rathinavelu, M. Dauphinee, and J. H. Fallon, "IRIDIUM exposure increases c-fos expression in the mouse brain only at levels which likely result in tissue heating," Neuroscience, Vol. 92, 1539-1546, 1999.
doi:10.1016/S0306-4522(99)00091-3 Google Scholar

21. Cullinan, W. E., J. P. Herman, D. F. Battaglia, H. Akil, and S. J. Watson, "Pattern and time course of immediate early gene expression in rat brain following acute stress," Neuroscience, Vol. 64, No. 2, 477-505, 1995.
doi:10.1016/0306-4522(94)00355-9 Google Scholar

22. Mausset-Bonnefont, A. L., H. Hirbec, X. Bonnefont, A. Privat, J. Vignon, and R. de Seze, "Acute exposure to GSM 900-MHz electromagnetic fields induces glial reactivity and biochemical modifications in the rat brain," Neurobiology of Disease, Vol. 17, 445-454, 2004.
doi:10.1016/j.nbd.2004.07.004 Google Scholar

Dr. Maria Elena Lopez-Martin

Dr. Julio C. Bregains

Dr. F. J. Jorge-Barreiro

Professor Jose Luis Sebastian Franco

Dr. Eduardo Moreno-Piquero

Professor Francisco Ares-Pena