volume | PIER Journals

Abstract

We perform the long time monitoring of nanoparticle-cell membrane interaction with high spatial and temporal resolution. The 2,3-bis(4-(phenyl(4-(1,2,2-triphenylvinyl) phenyl)amino)phenyl) fumaronitrile (TPE-TPA-FN) is doped in organically modified silica (ORMOSIL) to be a biocompatible nanoprobe, which displays an aggregation-induced emission (AIE) effect. Photobleaching resistance of this synthesized nanoparticle is tested and compared with its similar counterpart, which proves its superiority and capability of long term fluorescence emission. We utilize the objective-based total internal reflection microscopy combined with the living cell incubation platform to investigate the cell uptake process of this nanoparticle in real time.

1. Birks, J. B., Photophysics of Aromatic Molecules, 1970.

2. Qin, W., D. Ding, J. Z. Liu, W. Z. Yuan, Y. Hu, B. Liu, and B. Z. Tang, "Biocompatible nanoparticles with aggregation-induced emission characteristics as far-red/near-infrared fluorescent bioprobes for in vitro and in vivo imaging applications," Adv. Funct. Mater., Vol. 22, No. 4, 771-779, 2012.
doi:10.1002/adfm.201102191 Google Scholar

3. Yuan, W. Z., P. Lu, S. M. Chen, J. W. Y. Lam, Z. M. Wang, Y. Liu, H. S. Kwok, Y. G. Ma, and B. Z. Tang, "Changing the behavior of chromophores from aggregation-caused quenching to aggregation-induced emission: Development of highly efficient light emitters in the solid state," Adv. Mater., Vol. 22, No. 19, 2159, 2010.
doi:10.1002/adma.200904056 Google Scholar

4. Luo, J. D., Z. L. Xie, J. W. Y. Lam, L. Cheng, H. Y. Chen, C. F. Qiu, H. S. Kwok, X. W. Zhan, Y. Q. Liu, D. B. Zhu, and B. Z. Tang, "Aggregation-induced emission of 1-methyl-1, 2, 3, 4, 5-pentaphenylsilole," Chem. Commun., Vol. 18, 1740-1741, 2001.
doi:10.1039/b105159h Google Scholar

5. Hong, Y. N., J. W. Y. Lam, and B. Z. Tang, "Aggregation-induced emission: Phenomenon, mechanism and applications," Chem. Commun., Vol. 29, 4332-4353, 2009.
doi:10.1039/b904665h Google Scholar

6. Yu, Y., Y. N. Hong, C. Feng, J. Z. Liu, J. W. Y. Lam, M. T. Faisal, K. M. Ng, K. Q. Luo, and B. Z. Tang, "Synthesis of an AIE-active fluorogen and its application in cell imaging," Sci. China Ser. B, Vol. 52, No. 1, 15-19, 2009.
doi:10.1007/s11426-009-0008-0 Google Scholar

7. Axelrod, D., "Total internal reflection fluorescence microscopy in cell biology," Traffic, Vol. 2, No. 11, 764-774, 2001.
doi:10.1034/j.1600-0854.2001.21104.x Google Scholar

8. Jaiswal, J. K. and S. M. Simon, "Imaging single events at the cell membrane," Nat. Chem. Biol., Vol. 3, No. 2, 92-98, 2007.
doi:10.1038/nchembio855 Google Scholar

9. Ruthardt, N., D. C. Lamb, and C. Brauchle, "Single-particle tracking as a quantitative microscopy-based approach to unravel cell entry mechanisms of viruses and pharmaceutical nanoparticles," Mol. Ther., Vol. 19, No. 7, 1199-1211, 2011.
doi:10.1038/mt.2011.102 Google Scholar

10. Thompson, N. L. and B. L. Steele, "Total internal reflection with fluorescence correlation spectroscopy," Nat. Protoc., Vol. 2, No. 4, 878-890, 2007.
doi:10.1038/nprot.2007.110 Google Scholar

11. Toomre, D. and J. Bewersdorf, "A new wave of cellular imaging," Annu. Rev. Cell Dev. Bi., Vol. 26, 285-314, 2010.
doi:10.1146/annurev-cellbio-100109-104048 Google Scholar

12. Li, K., W. Qin, D. Ding, N. Tomczak, J. L. Geng, R. R. Liu, J. Z. Liu, X. H. Zhang, H. W. Liu, B. Liu, and B. Z. Tang, "Photostable fluorescent organic dots with aggregation-induced emission (AIE dots) for noninvasive long-term cell tracing," Sci. Rep., Vol. 3, UK, 2013. Google Scholar

13. Wang, D., J. Qian, S. L. He, J. S. Park, K. S. Lee, S. H. Han, and Y. Mu, "Aggregation-enhanced fluorescence in PEGylated phospholipid nanomicelles for in vivo imaging," Biomaterials, Vol. 32, No. 25, 5880-5888, 2011.
doi:10.1016/j.biomaterials.2011.04.080 Google Scholar

14. Qian, J., X. Li, M. Wei, X. W. Gao, Z. P. Xu, and S. L. He, "Bio-molecule-conjugated fluorescent organically modified silica nanoparticles as optical probes for cancer cell imaging," Opt. Express, Vol. 16, No. 24, 19568-19578, 2008.
doi:10.1364/OE.16.019568 Google Scholar

15. Lotito, V., U. Sennhauser C. V. Hafner, and G.-L. Bona, "Interaction of an asymmetric scanning near field optical microscopy probe with fluorescent molecules," Progress In Electromagnetics Research, Vol. 121, 281-299, 2011.
doi:10.2528/PIER11091703 Google Scholar

16. Bohmer, M. and J. Enderlein, "Orientation imaging of single molecules by wide-field epifluorescence microscopy," J. Opt. Soc. Am. B, Vol. 20, No. 3, 554-559, 2003.
doi:10.1364/JOSAB.20.000554 Google Scholar

17. Roy, R., S. Hohng, and T. Ha, "A practical guide to single-molecule FRET," Nat. Methods, Vol. 5, No. 6, 507-516, 2008.
doi:10.1038/nmeth.1208 Google Scholar

18. Gustafsson, M. G. L., "Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution," Pro. Natl. Acad. Sci. USA, Vol. 102, No. 37, 13081-13086, 2005.
doi:10.1073/pnas.0406877102 Google Scholar

19. Rust, M. J., M. Bates, and X. W. Zhuang, "Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)," Nat. Methods, Vol. 3, No. 10, 793-795, 2006.
doi:10.1038/nmeth929 Google Scholar

Dr. Hao Cheng

Mr. Wei Qin

Dr. Zhen Feng Zhu

Dr. Jun Qian

Dr. Anjun Qin

Dr. Ben Zhong Tang

Prof. Sailing He