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2014-03-28
Setup for Simultaneous Microwave Heating and Real-Time Spectrofluorometric Measurements in Biological Systems
By
Progress In Electromagnetics Research, Vol. 145, 229-240, 2014
Abstract
In this paper, a delivery system allowing simultaneous microwave heating and real-time spectrofluorometric measurements in biological systems is proposed and characterized. This system is used to investigate the phase behavior of lipid bilayers from about 15°C to 45°C. The delivery system is based on an open transverse electromagnetic (TEM) cell combined with a spectrofluorometer via an optical cable system. A numerical and experimental dosimetry of the delivery system is conducted. The Specific Absorption Rate (SAR) efficiency of the system is 26.1±2.1 W/kg/W. Spectrofluorometric measurements on Laurdan labeled small unilamellar vesicles (SUVs) are carried out. Generalized polarization (GP) of the SUV' membrane is obtained from the fluorescence intensities measured at two emission wavelengths.
Citation
Sophie Kohler, Nicolas Ticaud, Maria-Minodora Iordache, Mihaela G. Moisescu, Tudor Savopol, Philippe Leveque, and Delia Arnaud-Cormos, "Setup for Simultaneous Microwave Heating and Real-Time Spectrofluorometric Measurements in Biological Systems," Progress In Electromagnetics Research, Vol. 145, 229-240, 2014.
doi:10.2528/PIER13110703
References

1. Jones, D. A., T. P. Lelyveld, S. D. Mavrofidis, S. W. Kingman, and N. J. Miles, "Microwave heating applications in environmental engineering --- A review," Resources Conservation and Recycling, Vol. 34, 75-90, Jan. 2002.
doi:10.1016/S0921-3449(01)00088-X

2. Kappe, C. O., "Controlled microwave heating in modern organic synthesis," Angewandte Chemie-International Edition, Vol. 43, 6250-6284, 2004.
doi:10.1002/anie.200400655

3. Kappe, C. O. and D. Dallinger, "Controlled microwave heating in modern organic synthesis: Highlights from the 2004{2008 literature," Molecular Diversity, Vol. 13, 71-193, May 2009.
doi:10.1007/s11030-009-9138-8

4. Mahrour, N., R. Pologea-Moraru, M. G. Moisescu, S. Orlowski, P. Leveque, and L. M. Mir, "In vitro increase of the °uid-phase endocytosis induced by pulsed radiofrequency electromagnetic fields: importance of the electric field component," Biochimica Et Biophysica Acta-biomembranes,, Vol. 1668, 126-137, Feb. 2005.
doi:10.1016/j.bbamem.2004.11.015

5. Edidin, M., "Timeline --- Lipids on the frontier: A century of cell-membrane bilayers," Nature Reviews Molecular Cell Biology, Vol. 4, 414-418, May 2003.
doi:10.1038/nrm1102

6. Parasassi, T., G. De Stasio, A. Dubaldo, and E. Gratton, "Phase fluctuation in phospholipid-membranes revealed by laurdan fluorescence," Biophysical Journal, Vol. 57, 1179-1186, Jun. 1990.
doi:10.1016/S0006-3495(90)82637-0

7. Parasassi, T., G. De Stasio, G. Ravagnan, R. M. Rusch, and E. Gratton, "Quantitation of lipid phases in phospholipid-vesicles by the generalized polarization of laurdan fluorescence," Biophysical Journal, Vol. 60, 179-189, Jul. 1991.
doi:10.1016/S0006-3495(91)82041-0

8. Paffi, A., F. Apollonio, G. A. Lovisolo, C. Marino, R. Pinto, M. Repacholi, et al. "Considerations for developing an RF exposure system: A review for in vitro biological experiments," IEEE Transactions on Microwave Theory and Techniques, Vol. 58, 2702-2714, Oct. 2010.
doi:10.1109/TMTT.2010.2065351

9. Liberti, M., F. Apollonio, A. Pa±, M. Pellegrino, and G. D'Inzeo, "A coplanar-waveguide system for cells exposure during electrophysiological recordings," IEEE Transactions on Microwave Theory and Techniques, Vol. 52, 2521-2528, 2004.
doi:10.1109/TMTT.2004.837155

10. Paffi, A., M. Pellegrino, R. Beccherelli, F. Apollonio, M. Liberti, D. Platano, et al. "A real-time exposure system for electrophysiological recording in brain slices," IEEE Transactions on Microwave Theory and Techniques, Vol. 55, 2463-2471, 2007.
doi:10.1109/TMTT.2007.908657

11. Koester, P., J. Sakowski, W. Baumann, H.-W. Glock, and J. Gimsa, "A new exposure system for the in vitro detection of GHz field effects on neuronal networks," Bioelectrochemistry, Vol. 70, 104-114, 2007.
doi:10.1016/j.bioelechem.2006.03.039

12. Lambrecht, M. R., I. Chatterjee, D. McPherson, J. Quinn, T. Hagan, and G. L. Craviso, "Design, characterization, and optimization of a waveguide-based RF/MW exposure system for studying nonthermal e®ects on skeletal muscle contraction," IEEE Transactions on Plasma Science, Vol. 34, 1470-1479, 2006.
doi:10.1109/TPS.2006.876490

13. Ramundo-Orlando, A., M. Liberti, G. Mossa, and G. D'Inzeo, "Effects of 2.45 GHz microwave fields on liposomes entrapping glycoenzyme ascorbate oxidase: Evidence for oligosaccharide side chain involvement," Bioelectromagnetics, Vol. 25, 338-345, 2004.
doi:10.1002/bem.10203

14. Kenaan, M., M. G. Moisescu, T. Savopol, D. Martin, D. Arnaud-Cormos, and P. Leveque, "Dosimetry of an in vitro exposure system for fluorescence measurements during 2.45 GHz microwave exposure," International Journal of Microwave and Wireless Technologies, Vol. 3, 81-86, Feb. 2011.
doi:10.1017/S1759078710000784

15. Kohler, S., R. P. O'Connor, V. Thi Dan Thao, P. Leveque, and D. Arnaud-Cormos, "Experimental microdosimetry techniques for biological cells exposed to nanosecond pulsed electric fields using microfluorimetry," IEEE Transactions on Microwave Theory and Techniques, Vol. 61, 2015-2022, 2013.
doi:10.1109/TMTT.2013.2252917

16. Ticaud, N., S. Kohler, P. Jarrige, L. Duvillaret, G. Gaborit, R. P. O'Connor, et al. "Specific absorption rate assessment using simultaneous electric field and temperature measurements," IEEE Antennas and Wireless Propagation Letters, Vol. 11, 252-255, 2012.
doi:10.1109/LAWP.2012.2189748

17. Merla, C., N. Ticaud, D. Arnaud-Cormos, B. Veyret, and P. Leveque, "Real-time RF exposure setup based on a multiple electrode array (MEA) for electrophysiological recording of neuronal networks," IEEE Transactions on Microwave Theory and Techniques, Vol. 59, 755-762, Mar. 2011.
doi:10.1109/TMTT.2010.2100404

18. O'Connor, R. P., S. D. Madison, P. Leveque, H. L. Roderick, and . D. Bootman, "Exposure to GSM RF fields does not affect calcium homeostasis in human endothelial cells, rat pheocromocytoma cells or rat hippocampal neurons," Plos. One, Vol. 5, 16, Jul. 2010.

19. Moisescu, M. G., P. Leveque, J.-R. Bertrand, E. Kovacs, and L. M. Mir, "Microscopic observation of living cells during their exposure to modulated electromagnetic fields," 19th Biannual International Symposium on Bioelectrochemistry and Bioenergetics, 9-15, Toulouse, France, 2007.

20. Taflove, A. and S. C. Hagness, Computational Electrodynamics: The Finite-difference Time-domain Method, 3rd Ed., Artech House, Boston, 2005.

21. Yee, K., "Numerical solution of initial boundary value problems involving Maxwell's equations in isotropic media," IEEE Transactions on Antennas and Propagation, Vol. 14, 302-307, 1966.
doi:10.1109/TAP.1966.1138693

22. Leveque, P., A. Reineix, and B. Jecko, "Modeling of dielectric losses in microstrip patch antennas --- Application of FDTD method," Electronics Letters, Vol. 28, 539-541, Mar. 1992.
doi:10.1049/el:19920340

23. Melon, C., P. Leveque, T. Monediere, A. Reineix, and F. Jecko, "Frequency-dependent finite-difference time-domain [(FD)(2)TD] formulation applied to ferrite material," Microwave and Optical Technology Letters, Vol. 7, 577-579, Aug. 1994.
doi:10.1002/mop.4650071214

24. Berenger, J. P., "A perfectly matched layer for the absorption of electromagnetic-waves," Journal of Computational Physics, Vol. 114, 185-200, Oct. 1994.
doi:10.1006/jcph.1994.1159

25. McKinlay, A. F., J. H. Bernhardt, A. Ahlbom, U. Bergqvist, J. P. Cesarini, M. Grandolfo et al. "Guidance on determining compliance of exposure to pulsed and complex non-sinusoidal waveforms below 100 kHz with ICNIRP guidelines," Health Physics, Vol. 84, 383-387, 2003.
doi:10.1097/00004032-200303000-00016

26. Cueille, M., A. Collin, C. Pivain, and P. Leveque, "Development of a numerical model connecting electromagnetism, thermal and hydrodynamics to analyse in vitro exposure system," Annales Des Telecommunications --- Annals of Telecommunications, Vol. 63, 17-28, Feb. 2008.
doi:10.1007/s12243-007-0007-0

27. Kovacs, E., T. Savopol, M. M. Iordache, L. Saplacan, I. Sobaru, C. Istrate et al. "Interaction of gentamicin polycation with model and cell membranes," Bioelectrochemistry, Vol. 87, 230-235, Oct. 2012.
doi:10.1016/j.bioelechem.2012.03.001

28. Schuderer, J., D. Spat, T. Samaras, W. Oesch, and N. Kuster, "In vitro exposure systems for RF exposures at 900 MHz," IEEE Transactions on Microwave Theory and Techniques, Vol. 52, 2067-2075, 2004.
doi:10.1109/TMTT.2004.832010