volume | PIER Journals

Abstract

In this study, we introduce a dual-modal fluorescence hyperspectral micro-CT system developed for e.g. bioimaging applications. The system integrates an X-ray computed tomography (CT) module with a custom-designed hyperspectral fluorescence imaging module, achieving high-resolution structural imaging with detailed molecular-level insights. With a spectral resolution of 10 nm across the wavelength range of 450–750 nm, the hyperspectral fluorescence imaging module enables a fine compositional analysis. Using surface-modified nanoparticles, we demonstrate the system's capability to capture fluorescence under both X-ray and UV excitation. Imaging experiments on a mouse model further highlight the system's ability to generate comprehensive Four-dimensional (4D) datasets that integrate spatial, spectral, and structural information. To the best of our knowledge, no such a dual-modal system or the like has even been reported before. This dual-modal approach enhances the visualization and analysis of biological tissues, offering promising applications in e.g. disease diagnosis, surgical guidance, and preclinical research.

1. Jaju, Prashant P. and Sushma P. Jaju, "Clinical utility of dental cone-beam computed tomography: Current perspectives," Clinical, Cosmetic and Investigational Dentistry, Vol. 6, 29-43, 2014. Google Scholar

2. Cnudde, Veerle and Matthieu Nicolaas Boone, "High-resolution X-ray computed tomography in geosciences: A review of the current technology and applications," Earth-Science Reviews, Vol. 123, 1-17, 2013. Google Scholar

3. Schenck, John F., "The role of magnetic susceptibility in magnetic resonance imaging: MRI magnetic compatibility of the first and second kinds," Medical Physics, Vol. 23, No. 6, 815-850, 1996. Google Scholar

4. Edelman, R. R. and S. Warach, "Medical progress .1. magnetic-resonance-imaging," New England Journal of Medicine, Vol. 328, No. 10, 708-716, 1993. Google Scholar

5. Muehllehner, Gerd and Joel S. Karp, "Positron emission tomography," Physics in Medicine & Biology, Vol. 51, No. 13, R117, 2006. Google Scholar

6. Pahl, Jorg J., John C. Mazziotta, George Bartzokis, Jeffrey Cummings, Lori Altschuler, Jim Mintz, Stephen R. Marder, and Michael E. Phelps, "Positron-emission tomography in tardive dyskinesia," The Journal of Neuropsychiatry and Clinical Neurosciences, Vol. 7, No. 4, 457-465, 1995. Google Scholar

7. Han, Xiangjun, Ke Xu, Olena Taratula, and Khashayar Farsad, "Applications of nanoparticles in biomedical imaging," Nanoscale, Vol. 11, No. 3, 799-819, 2019. Google Scholar

8. Judenhofer, Martin S. and Simon R. Cherry, "Applications for preclinical PET/MRI," Seminars in Nuclear Medicine, Vol. 43, No. 1, 19-29, 2013.

9. Webb, W. R., M. G. Stein, W. E. Finkbeiner, J. G. Im, D. Lynch, and G. Gamsu, "Normal and diseased isolated lungs: high-resolution CT," Radiology, Vol. 166, No. 1, 81-87, 1988. Google Scholar

10. Maire, Eric and Philip John Withers, "Quantitative X-ray tomography," International Materials Reviews, Vol. 59, No. 1, 1-43, 2014. Google Scholar

11. Vásárhelyi, L., Z. Kónya, Á. Kukovecz, and R. Vajtai, "Microcomputed tomography-based characterization of advanced materials: A review," Materials Today Advances, Vol. 8, 100084, 2020. Google Scholar

12. Travincas, Rafael, Manuel F. C. Pereira, Isabel Torres, António Maurício, Dora Silveira, and Inês Flores-Colen, "X-ray microtomography applied to mortars: Review of microstructural visualization and parameterization," Micron, Vol. 164, 103375, 2023. Google Scholar

13. Park, Chan Ho, Zachary R. Abramson, Mario Taba Jr., Qiming Jin, Jia Chang, Jaclynn M. Kreider, Steven A. Goldstein, and William V. Giannobile, "Three-dimensional micro-computed tomographic imaging of alveolar bone in experimental bone loss or repair," Journal of Periodontology, Vol. 78, No. 2, 273-281, 2007. Google Scholar

14. Bouxsein, Mary L., Stephen K. Boyd, Blaine A. Christiansen, Robert E. Guldberg, Karl J. Jepsen, and Ralph Müller, "Guidelines for assessment of bone microstructure in rodents using micro-computed tomography," Journal of Bone and Mineral Research, Vol. 25, No. 7, 1468-1486, 2010. Google Scholar

15. Xu, Zhanpeng, Conor S. Locke, Richard Morris, DeAndre Jamison, Kenneth M. Kozloff, and Xueding Wang, "Development of a semi‐anthropomorphic photoacoustic calcaneus phantom based on nano computed tomography and stereolithography 3D printing," Journal of Orthopaedic Research, Vol. 42, No. 3, 647-660, 2024. Google Scholar

16. Eberspächer-Schweda, Matthias C., Kira Schmitt, Stephan Handschuh, Andrea Fuchs-Baumgartinger, and Alexander M. Reiter, "Diagnostic Yield of Micro-Computed tomography (micro-CT) versus histopathology of a canine oral fibrosarcoma," Journal of Veterinary Dentistry, Vol. 37, No. 1, 14-21, 2020. Google Scholar

17. Yang, Xi, Xue-Jun Huang, Zhang Chen, Ai-Li Xu, Hua Zhou, Xiao-Li Bi, Pei-Yu Yan, and Ying Xie, "A novel quantification method of lung fibrosis based on Micro-CT images developed with the optimized pulmonary fibrosis mice model induced by bleomycin," Heliyon, Vol. 9, No. 3, e13598, 2023. Google Scholar

18. Nebuloni, Laura, Gisela A. Kuhn, and Ralph Müller, "A comparative analysis of water-soluble and blood-pool contrast agents for in vivo vascular imaging with micro-CT," Academic Radiology, Vol. 20, No. 10, 1247-1255, 2013. Google Scholar

19. Faraj, Kaeuis A., Vincent M. J. I. Cuijpers, Ronnie G. Wismans, X. Frank Walboomers, John A. Jansen, Toin H. van Kuppevelt, and Willeke F. Daamen, "Micro-computed tomographical imaging of soft biological materials using contrast techniques," Tissue Engineering Part C: Methods, Vol. 15, No. 3, 493-499, 2009. Google Scholar

20. Dierick, Manuel, Veerle Cnudde, Bert Masschaele, Jelle Vlassenbroeck, Luc Van Hoorebeke, and Patric Jacobs, "Micro-CT of fossils preserved in amber," Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 580, No. 1, 641-643, 2007. Google Scholar

21. Şık, Alp, Hamed Tanabi, H, Evren Çubukçu, and Baris Sabuncuoglu, "Experimental and analytical investigation of the tensile behavior of 3D-printed composites based on micro-CT analysis," Journal of Thermoplastic Composite Materials, Vol. 37, No. 7, 2356-2376, 2024. Google Scholar

22. Clark, D. P. and C. T. Badea, "Advances in micro-CT imaging of small animals," Physica Medica, Vol. 88, 175-192, 2021. Google Scholar

23. Luo, Jing, Shuo Li, Erik Forsberg, and Sailing He, "4D surface shape measurement system with high spectral resolution and great depth accuracy," Optics Express, Vol. 29, No. 9, 13048-13070, 2021. Google Scholar

24. Lu, Guolan and Baowei Fei, "Medical hyperspectral imaging: A review," Journal of Biomedical Optics, Vol. 19, No. 1, 010901, 2014. Google Scholar

25. Xu, Zhanpeng, Erik Forsberg, Yang Guo, Fuhong Cai, and Sailing He, "Light-sheet microscopy for surface topography measurements and quantitative analysis," Sensors, Vol. 20, No. 10, 2842, 2020. Google Scholar

26. Yao, Xinli, Shuo Li, and Sailing He, "Dual-mode hyperspectral bio-imager with a conjugated camera for quick object-selection and focusing," Progress In Electromagnetics Research, Vol. 168, 133-143, 2020. Google Scholar

27. Zhu, Siqi, Kang Su, Yumeng Liu, Hao Yin, Zhen Li, Furong Huang, Zhenqiang Chen, Weidong Chen, Ge Zhang, and Yihong Chen, "Identification of cancerous gastric cells based on common features extracted from hyperspectral microscopic images," Biomedical Optics Express, Vol. 6, No. 4, 1135-1145, 2015. Google Scholar

28. Zhu, He, Jing Luo, Jiaqi Liao, and Sailing He, "High-accuracy rapid identification and classification of mixed bacteria using hyperspectral transmission microscopic imaging and machine learning," Progress In Electromagnetics Research, Vol. 178, 49-62, 2023. Google Scholar

29. Kim, Eun Bin, Yoo Sang Baek, and Onseok Lee, "A study on the classification of atopic dermatitis by spectral features of hyperspectral imaging," IEEE Access, 2024.
doi:10.1109/ACCESS.2024.3482557 Google Scholar

30. Ju, Qiang, Shouhua Luo, Chunxiao Chen, Zhenlan Fang, Shengkai Gao, Gong Chen, Xueyuan Chen, and Ning Gu, "Single-irradiation simultaneous dual-modal bioimaging using nanostructure scintillators as single contrast agent," Advanced Healthcare Materials, Vol. 8, No. 9, 1801324, 2019. Google Scholar

31. Xu, Zhanpeng, Wei Zhang, Carole Quesada, Xueding Wang, and Mario Fabiilli, "Longitudinal monitoring of angiogenesis in a murine window chamber model in vivo," Tissue Engineering Part C: Methods, Vol. 30, No. 3, 93-101, 2024. Google Scholar

32. Janjua, Raheel Ahmed, Chan Gao, Rucheng Dai, Zhilei Sui, Muhammad Aqeel Ahmad Raja, Zhongping Wang, Xianxu Zhen, and Zengming Zhang, "Na+-driven nucleation of NaYF4: Yb,er nanocrystals and effect of temperature on their structural transformations and luminescent properties," The Journal of Physical Chemistry C, Vol. 122, No. 40, 23242-23250, 2018. Google Scholar

33. Wang, Feng, Renren Deng, and Xiaogang Liu, "Preparation of core-shell NaGdF4 nanoparticles doped with luminescent lanthanide ions to be used as upconversion-based probes," Nature Protocols, Vol. 9, No. 7, 1634-1644, 2014. Google Scholar

Dr. Jing Luo

Ms. He Zhu

Dr. Raheel Ahmed Janjua

Dr. Wenbin Ji

Dr. Ruili Zhang

Dr. Junbo Liang

Prof. Sailing He